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her2  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc her2

    Her2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 495 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/erbb2/HER2%2FErbB2+Rabbit+mAb/pmc13669479-6-0-7
    Average 96 stars, based on 495 article reviews
    her2 - by Bioz Stars, 2026-11
    96/100 stars

    Images

    1) Product Images from "Fc‐Dependent Targeting of the GAS6–MERTK Axis With Monoclonal and Bispecific Antibodies Overcomes Drug Resistance Across EGFR Mutations in Exons 19, 20, and 21"

    Article Title: Fc‐Dependent Targeting of the GAS6–MERTK Axis With Monoclonal and Bispecific Antibodies Overcomes Drug Resistance Across EGFR Mutations in Exons 19, 20, and 21

    Journal: Advanced Science

    doi: 10.1002/advs.78290


    Figure Legend Snippet:

    Techniques Used: Cell-Signaling

    Related Articles

    Saline:

    Article Title: Polypharmacology‐Driven Discovery of ZAK‐I‐57: A Potent Multi‐Targeted Benzoxazinone Small Molecule for Hepatocellular Carcinoma Therapy
    Article Snippet: .. Membranes were blocked with 5% non‐fat milk in Tris‐buffered saline Tween 20 (TBST) for 1 h. The blots were incubated with primary antibodies (EGFR [4267S, Cell Signaling Technology, MA, USA], c‐Myc [5605S, Cell Signaling Technology], Bax [2772S, Cell Signaling Technology], Bcl‐2 [sc‐7382, Santa Cruze, TX, USA], ERBB2 [2165S, Cell Signaling Technology], and α‐tubulin [62204, Invitrogen, MA, USA], 1:1000) at 4°C overnight. .. Subsequently, the membranes were washed thrice (15 min each) with TBST solution and incubated for 1 h with secondary horseradish peroxidase‐conjugated antibodies (NA934 and NA931, Cytiva, MA, USA, 1:5000).

    Incubation:

    Article Title: Polypharmacology‐Driven Discovery of ZAK‐I‐57: A Potent Multi‐Targeted Benzoxazinone Small Molecule for Hepatocellular Carcinoma Therapy
    Article Snippet: .. Membranes were blocked with 5% non‐fat milk in Tris‐buffered saline Tween 20 (TBST) for 1 h. The blots were incubated with primary antibodies (EGFR [4267S, Cell Signaling Technology, MA, USA], c‐Myc [5605S, Cell Signaling Technology], Bax [2772S, Cell Signaling Technology], Bcl‐2 [sc‐7382, Santa Cruze, TX, USA], ERBB2 [2165S, Cell Signaling Technology], and α‐tubulin [62204, Invitrogen, MA, USA], 1:1000) at 4°C overnight. .. Subsequently, the membranes were washed thrice (15 min each) with TBST solution and incubated for 1 h with secondary horseradish peroxidase‐conjugated antibodies (NA934 and NA931, Cytiva, MA, USA, 1:5000).

    Western Blot:

    Article Title: N-phenylquinazolin-4-amine-based EGFR TKIs suppress pulmonary fibrosis by modulating the EGFR/ERBB3 axis in epithelial-macrophage interaction.
    Article Snippet: For quantification, aminimumof five images were acquired per section, and positively stained areas were measured with ImageJ software (NIH, Bethesda, MD; http:// imagej.net/). .. Antibodies for immunoblotting, immunofluorescence, and immunohistochemistry staining Immunoblotting, IHC, and IF staining were performed using primary antibodies against p-EGFR (IHC 1:100; immunoblotting 1:1000; Cell Signalling; #2234S), EGFR(IHC 1:100; immunoblotting 1:1000; Santa Cruz Biotechnology; #sc102), p-ERBB2 (IHC 1:200; immunoblotting 1:1000; Novusbio; #NB100-89474), ERBB2 (IHC 1:100; immunoblotting 1:1000; Cell Signalling; #2165), p-ERBB3 (IHC 1:200; immunoblotting 1:2000; Abcam; #ab131444), ERBB3 (IHC 1:100; Invitrogen; #MA513008), p-ERBB4 (IHC 1:200; immunoblotting 1:1000; Abcam; #ab61059), ERBB4 (IHC 1:200; immunoblotting 1:1000; Abcam; #ab19391), pro-SPC (IF 1:200; Merck; #AB3786), T1a (IF 1:200; R&D System; AF3244), arginase-1 (IHC/IF 1:2000; Novusbio; #NBP1-32731), iNOS (IHC/IF 1:100; Santa Cruz Biotechnology; #sc7271), TNF-α (IHC/ IF 1:100; R&D System; #AF-410NA), IL-6 (IHC/IF 1:200; Abcam; #ab208113), IFN-γ (IHC/IF 1:200; Abcam; #ab216642), EGF (IHC/IF 1:100; Santa Cruz Biotechnology; #sc166779), EREG (IHC/IF 1:100; Santa Cruz Biotechnology; #sc376284), NRG1 (IHC/IF 1:200; Invitrogen; #MA5-12896), AREG (IHC/IF 1:200; R&D System; #AF989), FSP (IF 1:200; Novus; NBP2-54580AF488), Fibronectin (IHC1:100; Abcam; #ab2413), MMP9 (IHC; 1:100 santa Cruz Biothechnology #sc6841), tdTomato (IF1:10,000; LSbio; #LS-C340696), and β-actin (immunoblotting 1:1000; Abcam; #ab8226). .. Actin stressfibers in cells were stainedwith Alexa Fluor 546-conjugated phalloidin (1:200; Invitrogen; #A22283), which specifically binds F-actin.

    Article Title: N-phenylquinazolin-4-amine-based EGFR TKIs suppress pulmonary fibrosis by modulating the EGFR/ERBB3 axis in epithelial–macrophage interaction
    Article Snippet: .. Immunoblotting, IHC, and IF staining were performed using primary antibodies against p-EGFR (IHC 1:100; immunoblotting 1:1000; Cell Signalling; #2234S), EGFR(IHC 1:100; immunoblotting 1:1000; Santa Cruz Biotechnology; #sc102), p-ERBB2 (IHC 1:200; immunoblotting 1:1000; Novusbio; #NB100-89474), ERBB2 (IHC 1:100; immunoblotting 1:1000; Cell Signalling; #2165), p-ERBB3 (IHC 1:200; immunoblotting 1:2000; Abcam; #ab131444), ERBB3 (IHC 1:100; Invitrogen; #MA5-13008), p-ERBB4 (IHC 1:200; immunoblotting 1:1000; Abcam; #ab61059), ERBB4 (IHC 1:200; immunoblotting 1:1000; Abcam; #ab19391), pro-SPC (IF 1:200; Merck; #AB3786), T1a (IF 1:200; R&D System; AF3244), arginase-1 (IHC/IF 1:2000; Novusbio; #NBP1-32731), iNOS (IHC/IF 1:100; Santa Cruz Biotechnology; #sc7271), TNF-α (IHC/IF 1:100; R&D System; #AF-410NA), IL-6 (IHC/IF 1:200; Abcam; #ab208113), IFN-γ (IHC/IF 1:200; Abcam; #ab216642), EGF (IHC/IF 1:100; Santa Cruz Biotechnology; #sc166779), EREG (IHC/IF 1:100; Santa Cruz Biotechnology; #sc376284), NRG1 (IHC/IF 1:200; Invitrogen; #MA5-12896), AREG (IHC/IF 1:200; R&D System; #AF989), FSP (IF 1:200; Novus; NBP2-54580AF488), Fibronectin (IHC1:100; Abcam; #ab2413), MMP9 (IHC; 1:100 santa Cruz Biothechnology #sc6841), tdTomato (IF1:10,000; LSbio; #LS-C340696), and β-actin (immunoblotting 1:1000; Abcam; #ab8226). .. Actin stress fibers in cells were stained with Alexa Fluor 546-conjugated phalloidin (1:200; Invitrogen; #A22283), which specifically binds F-actin.

    Immunofluorescence:

    Article Title: N-phenylquinazolin-4-amine-based EGFR TKIs suppress pulmonary fibrosis by modulating the EGFR/ERBB3 axis in epithelial-macrophage interaction.
    Article Snippet: For quantification, aminimumof five images were acquired per section, and positively stained areas were measured with ImageJ software (NIH, Bethesda, MD; http:// imagej.net/). .. Antibodies for immunoblotting, immunofluorescence, and immunohistochemistry staining Immunoblotting, IHC, and IF staining were performed using primary antibodies against p-EGFR (IHC 1:100; immunoblotting 1:1000; Cell Signalling; #2234S), EGFR(IHC 1:100; immunoblotting 1:1000; Santa Cruz Biotechnology; #sc102), p-ERBB2 (IHC 1:200; immunoblotting 1:1000; Novusbio; #NB100-89474), ERBB2 (IHC 1:100; immunoblotting 1:1000; Cell Signalling; #2165), p-ERBB3 (IHC 1:200; immunoblotting 1:2000; Abcam; #ab131444), ERBB3 (IHC 1:100; Invitrogen; #MA513008), p-ERBB4 (IHC 1:200; immunoblotting 1:1000; Abcam; #ab61059), ERBB4 (IHC 1:200; immunoblotting 1:1000; Abcam; #ab19391), pro-SPC (IF 1:200; Merck; #AB3786), T1a (IF 1:200; R&D System; AF3244), arginase-1 (IHC/IF 1:2000; Novusbio; #NBP1-32731), iNOS (IHC/IF 1:100; Santa Cruz Biotechnology; #sc7271), TNF-α (IHC/ IF 1:100; R&D System; #AF-410NA), IL-6 (IHC/IF 1:200; Abcam; #ab208113), IFN-γ (IHC/IF 1:200; Abcam; #ab216642), EGF (IHC/IF 1:100; Santa Cruz Biotechnology; #sc166779), EREG (IHC/IF 1:100; Santa Cruz Biotechnology; #sc376284), NRG1 (IHC/IF 1:200; Invitrogen; #MA5-12896), AREG (IHC/IF 1:200; R&D System; #AF989), FSP (IF 1:200; Novus; NBP2-54580AF488), Fibronectin (IHC1:100; Abcam; #ab2413), MMP9 (IHC; 1:100 santa Cruz Biothechnology #sc6841), tdTomato (IF1:10,000; LSbio; #LS-C340696), and β-actin (immunoblotting 1:1000; Abcam; #ab8226). .. Actin stressfibers in cells were stainedwith Alexa Fluor 546-conjugated phalloidin (1:200; Invitrogen; #A22283), which specifically binds F-actin.

    Immunohistochemistry:

    Article Title: N-phenylquinazolin-4-amine-based EGFR TKIs suppress pulmonary fibrosis by modulating the EGFR/ERBB3 axis in epithelial-macrophage interaction.
    Article Snippet: For quantification, aminimumof five images were acquired per section, and positively stained areas were measured with ImageJ software (NIH, Bethesda, MD; http:// imagej.net/). .. Antibodies for immunoblotting, immunofluorescence, and immunohistochemistry staining Immunoblotting, IHC, and IF staining were performed using primary antibodies against p-EGFR (IHC 1:100; immunoblotting 1:1000; Cell Signalling; #2234S), EGFR(IHC 1:100; immunoblotting 1:1000; Santa Cruz Biotechnology; #sc102), p-ERBB2 (IHC 1:200; immunoblotting 1:1000; Novusbio; #NB100-89474), ERBB2 (IHC 1:100; immunoblotting 1:1000; Cell Signalling; #2165), p-ERBB3 (IHC 1:200; immunoblotting 1:2000; Abcam; #ab131444), ERBB3 (IHC 1:100; Invitrogen; #MA513008), p-ERBB4 (IHC 1:200; immunoblotting 1:1000; Abcam; #ab61059), ERBB4 (IHC 1:200; immunoblotting 1:1000; Abcam; #ab19391), pro-SPC (IF 1:200; Merck; #AB3786), T1a (IF 1:200; R&D System; AF3244), arginase-1 (IHC/IF 1:2000; Novusbio; #NBP1-32731), iNOS (IHC/IF 1:100; Santa Cruz Biotechnology; #sc7271), TNF-α (IHC/ IF 1:100; R&D System; #AF-410NA), IL-6 (IHC/IF 1:200; Abcam; #ab208113), IFN-γ (IHC/IF 1:200; Abcam; #ab216642), EGF (IHC/IF 1:100; Santa Cruz Biotechnology; #sc166779), EREG (IHC/IF 1:100; Santa Cruz Biotechnology; #sc376284), NRG1 (IHC/IF 1:200; Invitrogen; #MA5-12896), AREG (IHC/IF 1:200; R&D System; #AF989), FSP (IF 1:200; Novus; NBP2-54580AF488), Fibronectin (IHC1:100; Abcam; #ab2413), MMP9 (IHC; 1:100 santa Cruz Biothechnology #sc6841), tdTomato (IF1:10,000; LSbio; #LS-C340696), and β-actin (immunoblotting 1:1000; Abcam; #ab8226). .. Actin stressfibers in cells were stainedwith Alexa Fluor 546-conjugated phalloidin (1:200; Invitrogen; #A22283), which specifically binds F-actin.

    Article Title: N-phenylquinazolin-4-amine-based EGFR TKIs suppress pulmonary fibrosis by modulating the EGFR/ERBB3 axis in epithelial–macrophage interaction
    Article Snippet: .. Immunoblotting, IHC, and IF staining were performed using primary antibodies against p-EGFR (IHC 1:100; immunoblotting 1:1000; Cell Signalling; #2234S), EGFR(IHC 1:100; immunoblotting 1:1000; Santa Cruz Biotechnology; #sc102), p-ERBB2 (IHC 1:200; immunoblotting 1:1000; Novusbio; #NB100-89474), ERBB2 (IHC 1:100; immunoblotting 1:1000; Cell Signalling; #2165), p-ERBB3 (IHC 1:200; immunoblotting 1:2000; Abcam; #ab131444), ERBB3 (IHC 1:100; Invitrogen; #MA5-13008), p-ERBB4 (IHC 1:200; immunoblotting 1:1000; Abcam; #ab61059), ERBB4 (IHC 1:200; immunoblotting 1:1000; Abcam; #ab19391), pro-SPC (IF 1:200; Merck; #AB3786), T1a (IF 1:200; R&D System; AF3244), arginase-1 (IHC/IF 1:2000; Novusbio; #NBP1-32731), iNOS (IHC/IF 1:100; Santa Cruz Biotechnology; #sc7271), TNF-α (IHC/IF 1:100; R&D System; #AF-410NA), IL-6 (IHC/IF 1:200; Abcam; #ab208113), IFN-γ (IHC/IF 1:200; Abcam; #ab216642), EGF (IHC/IF 1:100; Santa Cruz Biotechnology; #sc166779), EREG (IHC/IF 1:100; Santa Cruz Biotechnology; #sc376284), NRG1 (IHC/IF 1:200; Invitrogen; #MA5-12896), AREG (IHC/IF 1:200; R&D System; #AF989), FSP (IF 1:200; Novus; NBP2-54580AF488), Fibronectin (IHC1:100; Abcam; #ab2413), MMP9 (IHC; 1:100 santa Cruz Biothechnology #sc6841), tdTomato (IF1:10,000; LSbio; #LS-C340696), and β-actin (immunoblotting 1:1000; Abcam; #ab8226). .. Actin stress fibers in cells were stained with Alexa Fluor 546-conjugated phalloidin (1:200; Invitrogen; #A22283), which specifically binds F-actin.

    Staining:

    Article Title: N-phenylquinazolin-4-amine-based EGFR TKIs suppress pulmonary fibrosis by modulating the EGFR/ERBB3 axis in epithelial-macrophage interaction.
    Article Snippet: For quantification, aminimumof five images were acquired per section, and positively stained areas were measured with ImageJ software (NIH, Bethesda, MD; http:// imagej.net/). .. Antibodies for immunoblotting, immunofluorescence, and immunohistochemistry staining Immunoblotting, IHC, and IF staining were performed using primary antibodies against p-EGFR (IHC 1:100; immunoblotting 1:1000; Cell Signalling; #2234S), EGFR(IHC 1:100; immunoblotting 1:1000; Santa Cruz Biotechnology; #sc102), p-ERBB2 (IHC 1:200; immunoblotting 1:1000; Novusbio; #NB100-89474), ERBB2 (IHC 1:100; immunoblotting 1:1000; Cell Signalling; #2165), p-ERBB3 (IHC 1:200; immunoblotting 1:2000; Abcam; #ab131444), ERBB3 (IHC 1:100; Invitrogen; #MA513008), p-ERBB4 (IHC 1:200; immunoblotting 1:1000; Abcam; #ab61059), ERBB4 (IHC 1:200; immunoblotting 1:1000; Abcam; #ab19391), pro-SPC (IF 1:200; Merck; #AB3786), T1a (IF 1:200; R&D System; AF3244), arginase-1 (IHC/IF 1:2000; Novusbio; #NBP1-32731), iNOS (IHC/IF 1:100; Santa Cruz Biotechnology; #sc7271), TNF-α (IHC/ IF 1:100; R&D System; #AF-410NA), IL-6 (IHC/IF 1:200; Abcam; #ab208113), IFN-γ (IHC/IF 1:200; Abcam; #ab216642), EGF (IHC/IF 1:100; Santa Cruz Biotechnology; #sc166779), EREG (IHC/IF 1:100; Santa Cruz Biotechnology; #sc376284), NRG1 (IHC/IF 1:200; Invitrogen; #MA5-12896), AREG (IHC/IF 1:200; R&D System; #AF989), FSP (IF 1:200; Novus; NBP2-54580AF488), Fibronectin (IHC1:100; Abcam; #ab2413), MMP9 (IHC; 1:100 santa Cruz Biothechnology #sc6841), tdTomato (IF1:10,000; LSbio; #LS-C340696), and β-actin (immunoblotting 1:1000; Abcam; #ab8226). .. Actin stressfibers in cells were stainedwith Alexa Fluor 546-conjugated phalloidin (1:200; Invitrogen; #A22283), which specifically binds F-actin.

    Article Title: Recombinant Human Neuregulin1-β1 Significantly Reduces Schwannoma Growth in Mice.
    Article Snippet: .. A total of 4μm thick cross sections were used for hematoxylin and eosin (H&E) staining and immunohistochemical labeling of S100beta (S100b) (1:8000; Dako; GA50461-2), myelin protein zero (MPZ) (1:300; Bioss Antibodies; bs-0337R), p75 (1:200; Millipore; AB1554), and ErbB2 (1:500; Cell Signaling; 2,165) in an automated Ventana stainer (Ventana Medical Systems, USA), using standard antigen retrieval protocols (CC1st, no pretreatment for S100b-protein). .. Immunoblotting was completed as described in Morrison et al,19 using the following primary antibodies: c-Jun (1:1,000; Cell Signaling; 8,752), anti-ErbB2 (1:500; Cell Signaling; 2,165), anti-GAPDH (1:1,000; Santa Cruz; 6C5), myelin basic protein (MBP) (1:500; Millipore; MAB384), and anti-merlin (1:500; Santa Cruz; A19).

    Article Title: N-phenylquinazolin-4-amine-based EGFR TKIs suppress pulmonary fibrosis by modulating the EGFR/ERBB3 axis in epithelial–macrophage interaction
    Article Snippet: .. Immunoblotting, IHC, and IF staining were performed using primary antibodies against p-EGFR (IHC 1:100; immunoblotting 1:1000; Cell Signalling; #2234S), EGFR(IHC 1:100; immunoblotting 1:1000; Santa Cruz Biotechnology; #sc102), p-ERBB2 (IHC 1:200; immunoblotting 1:1000; Novusbio; #NB100-89474), ERBB2 (IHC 1:100; immunoblotting 1:1000; Cell Signalling; #2165), p-ERBB3 (IHC 1:200; immunoblotting 1:2000; Abcam; #ab131444), ERBB3 (IHC 1:100; Invitrogen; #MA5-13008), p-ERBB4 (IHC 1:200; immunoblotting 1:1000; Abcam; #ab61059), ERBB4 (IHC 1:200; immunoblotting 1:1000; Abcam; #ab19391), pro-SPC (IF 1:200; Merck; #AB3786), T1a (IF 1:200; R&D System; AF3244), arginase-1 (IHC/IF 1:2000; Novusbio; #NBP1-32731), iNOS (IHC/IF 1:100; Santa Cruz Biotechnology; #sc7271), TNF-α (IHC/IF 1:100; R&D System; #AF-410NA), IL-6 (IHC/IF 1:200; Abcam; #ab208113), IFN-γ (IHC/IF 1:200; Abcam; #ab216642), EGF (IHC/IF 1:100; Santa Cruz Biotechnology; #sc166779), EREG (IHC/IF 1:100; Santa Cruz Biotechnology; #sc376284), NRG1 (IHC/IF 1:200; Invitrogen; #MA5-12896), AREG (IHC/IF 1:200; R&D System; #AF989), FSP (IF 1:200; Novus; NBP2-54580AF488), Fibronectin (IHC1:100; Abcam; #ab2413), MMP9 (IHC; 1:100 santa Cruz Biothechnology #sc6841), tdTomato (IF1:10,000; LSbio; #LS-C340696), and β-actin (immunoblotting 1:1000; Abcam; #ab8226). .. Actin stress fibers in cells were stained with Alexa Fluor 546-conjugated phalloidin (1:200; Invitrogen; #A22283), which specifically binds F-actin.

    Immunohistochemical staining:

    Article Title: Recombinant Human Neuregulin1-β1 Significantly Reduces Schwannoma Growth in Mice.
    Article Snippet: .. A total of 4μm thick cross sections were used for hematoxylin and eosin (H&E) staining and immunohistochemical labeling of S100beta (S100b) (1:8000; Dako; GA50461-2), myelin protein zero (MPZ) (1:300; Bioss Antibodies; bs-0337R), p75 (1:200; Millipore; AB1554), and ErbB2 (1:500; Cell Signaling; 2,165) in an automated Ventana stainer (Ventana Medical Systems, USA), using standard antigen retrieval protocols (CC1st, no pretreatment for S100b-protein). .. Immunoblotting was completed as described in Morrison et al,19 using the following primary antibodies: c-Jun (1:1,000; Cell Signaling; 8,752), anti-ErbB2 (1:500; Cell Signaling; 2,165), anti-GAPDH (1:1,000; Santa Cruz; 6C5), myelin basic protein (MBP) (1:500; Millipore; MAB384), and anti-merlin (1:500; Santa Cruz; A19).

    Labeling:

    Article Title: Recombinant Human Neuregulin1-β1 Significantly Reduces Schwannoma Growth in Mice.
    Article Snippet: .. A total of 4μm thick cross sections were used for hematoxylin and eosin (H&E) staining and immunohistochemical labeling of S100beta (S100b) (1:8000; Dako; GA50461-2), myelin protein zero (MPZ) (1:300; Bioss Antibodies; bs-0337R), p75 (1:200; Millipore; AB1554), and ErbB2 (1:500; Cell Signaling; 2,165) in an automated Ventana stainer (Ventana Medical Systems, USA), using standard antigen retrieval protocols (CC1st, no pretreatment for S100b-protein). .. Immunoblotting was completed as described in Morrison et al,19 using the following primary antibodies: c-Jun (1:1,000; Cell Signaling; 8,752), anti-ErbB2 (1:500; Cell Signaling; 2,165), anti-GAPDH (1:1,000; Santa Cruz; 6C5), myelin basic protein (MBP) (1:500; Millipore; MAB384), and anti-merlin (1:500; Santa Cruz; A19).

    Control:

    Article Title: The ERBB2 c.1795C>T, p.Arg599Cys variant is associated with left ventricular outflow tract obstruction defects in humans
    Article Snippet: Simple Western was then performed using Jess Automated Western Blot system (004-650, Biotechne), 66-400 kDa Fluorescence Separation Module (SM-FL005, Biotechne), Anti-Rabbit Detection Module (DM-001, Biotechne) and Protein Normalization Module (DM-PN02, Biotechne). .. After 2 hous in RPMI without supplement, ERBB2 proband-hiPS-CMs and control hiPS-CMs (HEL47.2) were stimulated with Recombinant Human NRG1-beta 1 (18166920, Fisher Scientific) 100 ng/ml for 30 minutes. .. They were then lysed in RIPA buffer, centrifuged for 15 min, 12 000 g and supernatant collected and protein amount measured with Pierce BCA Protein Assay kit (23227, Thermo Scientific).

    Recombinant:

    Article Title: The ERBB2 c.1795C>T, p.Arg599Cys variant is associated with left ventricular outflow tract obstruction defects in humans
    Article Snippet: Simple Western was then performed using Jess Automated Western Blot system (004-650, Biotechne), 66-400 kDa Fluorescence Separation Module (SM-FL005, Biotechne), Anti-Rabbit Detection Module (DM-001, Biotechne) and Protein Normalization Module (DM-PN02, Biotechne). .. After 2 hous in RPMI without supplement, ERBB2 proband-hiPS-CMs and control hiPS-CMs (HEL47.2) were stimulated with Recombinant Human NRG1-beta 1 (18166920, Fisher Scientific) 100 ng/ml for 30 minutes. .. They were then lysed in RIPA buffer, centrifuged for 15 min, 12 000 g and supernatant collected and protein amount measured with Pierce BCA Protein Assay kit (23227, Thermo Scientific).

    other:

    Article Title: Metformin enhances alpelisib sensitivity in HER2+ breast cancer by suppressing cancer stemness and oncogenic signaling
    Article Snippet: Primary antibodies against phosphorylated ERK1/2 (Thr202/Tyr204) (p-ERK1/2, Cat: 9101), ERK1/2(Cat: 9102), phosphorylated Akt (Ser473) (p-Akt, Cat: 4060), Akt(Cat: 9272), phosphorylated Stat3 (Tyr705) (p-Stat3, Cat: 9145), Stat3 (Cat: 4904), Cyclin D1(Cat: 55506), c-Myc (Cat: 9402), phosphorylated ErbB2 (Tyr1221/1222) (p-ErbB2, Cat: 2243), ErbB2 (Cat: 2242), β-Catenin (Cat: 8480), Active β-Catenin (Cat: 19807), phosphorylated mTOR (Ser2448) (p-mTOR, Cat: 5536), mTOR (Cat: 2983), phosphorylated S6K (Thr389) (p-S6K, Cat: 9234), S6K (Cat: 9202), Nanog (Cat: 4903), KLF4 (Cat: 12173), Sox2 (Cat: 23064), AMPK (Cat: 2532), p-AMPK (Cat: 2535) along with Anti-mouse/rabbit HRP-linked secondary antibodies, were purchased from Cell Signaling Technology (Danvers, MA, USA).



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    Image Search Results


    Journal: Advanced Science

    Article Title: Fc‐Dependent Targeting of the GAS6–MERTK Axis With Monoclonal and Bispecific Antibodies Overcomes Drug Resistance Across EGFR Mutations in Exons 19, 20, and 21

    doi: 10.1002/advs.78290

    Figure Lengend Snippet:

    Article Snippet: HER2 , 2165S , Cell Signaling Technology (CST) , AB_10692490.

    Techniques: Cell-Signaling